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莱斯特城队夺冠:Calbindin D-9k

貨號 貨期 規格 / 價格 詢價

Calbindin D-9k

品牌

Swant

貨號

rCB9

產品分類

Recombinant Protein

研究領域

Calbindin

產品概述

Freeze in small aliquots (e.g. 1 or 10 ug) and keep at -80°C. Avoid repeated freezing and thawing.

濃度

Rat recombinant calbindin D-9k (recombinant) produced in Escherichia Coli.

純度

Recombinant rat

產品描述

Purification: Ni-chelate chrom. Lot No: 5 Reconstitution: 10 ug protein reconstituted with 25 ul PBS (0.4 ug/ul) 60 ug protein reconstituted with 150 ul PBS Purification: E. coli containing a His6-fusion protein were disrupted in a buffer containing 6M GuHCl/0.1 M NaH2PO4. After a low speed centrifugation to remove cell debris, the supernatant was applied to the charged NTA resin column, equilibrated in disruption buffer, before the column being washed with the same buffer. Afterwards, the column was washed and the HIS6- fusion proteins eluted by decreasing the pH of the eluent (8 M Urea /0.1 M Na2HPO4) in a stepwise manner (pH 6.3 / 5.9 / 4 / 2). The protein was dialysed against phosphate buffered saline, 0.1 M, pH 7.3 (PBS).

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數量

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